UPLC測(cè)定黃連中鹽酸小檗堿和鹽酸巴馬汀的含量
《中國(guó)測(cè)試》雜志
譚 鐳1，2， 詹 雁2， 阮 佳2， 徐超群2
（1.四川大學(xué)華西藥學(xué)院，四川 成都 610041；2.四川省中醫(yī)藥科學(xué)院，四川 成都 610041）
摘 要：建立UPLC測(cè)定黃連中鹽酸小檗堿和鹽酸巴馬汀含量的方法。采用ACQUITY UPLC BEH-C18色譜柱（2.1 mm×50 mm×1.7 μm），以乙腈-0.05 mol/L磷酸二氫鉀溶液為流動(dòng)相，梯度洗脫，流速為0.2 mL/min，檢測(cè)波長(zhǎng)為350 nm。結(jié)果表明鹽酸小檗堿和鹽酸巴馬汀分別在0.037 2～0.297 6 μg/mL，0.008 2～0.065 6 μg/mL的濃度范圍內(nèi)均有良好的線性關(guān)系（r=0.999 9），回收率均大于95%，RSD均小于2%。該方法快速、準(zhǔn)確、重現(xiàn)性好，可用于測(cè)定黃連中鹽酸小檗堿和鹽酸巴馬汀的含量。
關(guān)鍵詞：黃連；鹽酸小檗堿；鹽酸巴馬汀；超高效液相色譜法（UPLC）
中圖分類號(hào)：S567.5+2；TQ462+.12；R284.1；O657.31 文獻(xiàn)標(biāo)志碼：A 文章編號(hào)：1674-5124（2013）03-0054-03
UPLC simultaneous determination of berberine hydrochloride and
palmatine hydrochloride in Coptis
TAN Lei1，2， ZHAN Yan2， RUAN Jia2， XU Chao-qun2
（1.West China School of pharmacy，Sichuan University，Chengdu 610041，China；
2.Sichuan Academy of Chinese Medicine Sciences，Chengdu 610041，China）
Abstract: A method was established for simultaneously determining berberine hydrochloride and palmatine hydrochloride in Coptis by UPLC. The optimal conditions of separation and detection were achieved on an ACQUITY UPLC BEH-C18 column （2.1 mm×50 mm×1.7 μm） eluted with a gradient acetonitrile and 0.05 mol/L potassium dihydrogen phosphate solution at a flow rate of 0.2 mL/min， and detected at 350 nm. The results showed that there were good linear relationships （r=0.999 9） when the concentration of berberine hydrochloride and palmatine hydrochloride were in the range of 0.0372-0.2976 μg/mL and 0.0082-0.0656 μg/mL respectively， the recovery rates were more than 95%， and RSDs were less than 2%. This method was simple， rapid， accurate， and reproducible， and it can be used for determining the concentration of berberine hydrochloride and palmatine hydrochloride in Coptis.
Key words: Coptis； berberine hydrochloride； palmatine hydrochloride； UPLC；
